Use of dengue NS1 antigen for early diagnosis ...

Accurate and timely diagnosis of dengue virus is important for early detection of dengue virus infection. In this study, the usefulness of the dengue NS1 antigen test was evaluated as a routine, rapid diagnostic test for dengue virus infection. A total of 208 sera from patients suspected of having dengue virus infection were collected and tested for dengue antibody, dengue genome and dengue NS1 antigen. Dengue antibody test, dengue PCR test and dengue antigen test were able to detect dengue virus infection from Days 1 to 8 in 72.8, 52.8 and 44.0% of samples, respectively. Of the 208 sera tested, 69.2% (144/208) of the acute sera were positive for dengue virus infection based on IgM antibody, IgG antibody, NS1 antigen and PCR tests. Thirty-two point two percent of the samples (67/208) were found positive for dengue NS1 antigen, 38.5% (80/208) were PCR positive, 40.9% (85/208) were IgM positive and 36.1% (75/208) were IgG positive for dengue virus. The results reveal the detection rate of dengue virus infection was similar for PCR and dengue antibody (65.9%) and for NS1 antigen and dengue antibody (62.0%) combinations. Therefore, the dengue NS1 antigen test can be used to complement the current antibody test used in peripheral laboratories. Thus, the combination of the NS1 antigen and antibody tests could increase the diagnostic efficiency for early diagnosis of dengue infection.

For more dengue ns1 antigeninformation, please contact us. We will provide professional answers.

Test for dengue fever

Crystal structure of the dengue virus, which causes dengue fever

NS1 antigen test (nonstructural protein 1) is a test for dengue, introduced in 2006. It allows rapid detection on the first day of fever, before antibodies appear some 5 or more days later.[1] It has been adopted for use in some 40 nations. The method of detection is through enzyme-linked immunosorbent assay.[1][2] India has introduced in 2010 the NS1 test costing 1,600 rupees at a private hospital in Mumbai.[2]

Medical use

[

edit

]

The medical use of the NS1 antigen test can be defined to diagnose dengue infections and is effective to 1st day detection. Additionally, NS1 assay is useful for differential diagnostics in regards to flaviviruses.[3]

NS1 is present in the serum of infected persons directly at the onset of clinical symptoms in primary dengue infection and produces a strong humoral response. It is detectable before the appearance of IgM antibodies.[4]

DENV by NS1 antigen is laboratory confirmation of dengue in people also assessing clinical aspects (as well as, taking into account where the individual may have traveled recently).[5] Serological tests such as an immunoglobulin M antibody capture–enzyme-linked immunosorbent assay (MAC-ELISA) and viral RNA detection by reverse transcriptase (RT-PCR) can also be used to diagnose dengue fever.[6]

See also

Additional reading:
Home Test for Urinary Tract Infections

For more information, please visit Multi-Drug Urine Panel.

[

edit

]

References

[

edit

]

Further reading

[

edit

]

If you want to learn more, please visit our website alcohol strip.